Antidiuretic and antidiarrhoeal activities of polar and non-polar extract of Brassica oleracea.

Diuretic activities of both polar and non-polar extract of leaves of Brassica oleracea were investigated on male white rabbits and male Sprague-Dawley rats. Anti diarrheal activity of the same extract was investigated on male and female swiss albino mice. Both polar and non-polar extract exhibited anti diuretic activities on both rats and rabbits. Polar and Non-polar extract also showed anti diarrheal activity on male and female mice. Anti diarrheal activity affects both latent period and number of stools.

Antimicrobial and Cytotoxic Activity of the Methanol Extract of Paederia foetida Linn. (Rubiaceae).

The antibacterial activities of n-hexane, chloroform, ethyl acetate fractions of methanolic extracts of the whole plants Paederia foetida (family Rubiaceae) were screened against various pathogenic bacteria such as Bacillus cereus, Bacillus megaterium, Bacillus subtilis, Staphylococcus aureus, Sarcina lutea, Escherichia coli, Pseudomonas aeruginosa, Salmonella paratyphi , Salmonella typhi, Shigella boydii, Shigella dysenteriae, Vibrio mimicus, Vibrio parahemolyticus, Candida albicans, Aspergillus niger, Sacharomyces cerevacae by ‘disc diffusion method’. The methanol extract of the whole plants possesses no antimicrobial activity but the ethyl acetate, chloroform and n-hexane fractions exhibited moderate to less activity against some organisms tested compared with the standard antibiotic Kanamycin. Brine shrimp lethality bio-assay was done using brine shrimp Nauplii and dimethyl sulfoxide as a solvent for the methanol plant extracts of Paederia foetida. The LC50 value of methanol extract of the plant indicated that the cytotoxicity was very significant.

Study of binary and ternary solid dispersion of ibuprofen for the enhancement of oral bioavailability.

To develop a novel ibuprofen loaded solid dispersion system (SDs) with enhanced dissolution rate, binary and ternary solid dispersion were prepared by co-precipitation method using poloxamer-407 only and mixture of poloxamer-407 with a second polymer such as HPMC 6cps, HPC, Kollicoat IR, Kollidon VA 64 respectively. In case of binary SDs, poloxamer 407 was used in three concentrations: 33%, 50% and 66.67% wt/wt of total SDs. In case of ternary SDs, poloxamer 407 was used at 15%, 25% 35% wt/wt of the total SDs content while maintaining the concentration of the second polymer at fixed amount (1gm). In vitro dissolution study was conducted in phosphate buffer of pH 6.8 for 1h. Release property of ibuprofen from two different SDs was investigated. And in case of both the systems, enhanced release property was found where the release was compared with pure ibuprofen powder. Enhanced release of ibuprofen from the optimized SDs was characterized in light of cumulative percent release, % release after 5 min of dissolution and release rate of the drug from different SDs. When the amount of carriers increased with a decrease in drug content, the release of ibuprofen was elevated. And it was found that almost two fold increase in the release of ibuprofen while 66.67% poloxamer was used.